Shank3 phosphorylation
Webb29 apr. 2024 · In particular, the authors provide compelling evidence that the phosphorylation state of one synaptic scaffolding protein, Shank 3, is a necessary part … Webb26 mars 2024 · The synaptic scaffolding protein Shank3 binds CaMKII, ABI1, and many other proteins. We show that Ser685 in Shank3 can be phosphorylated by CaMKII or …
Shank3 phosphorylation
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Webb16 nov. 2024 · Notably, murine SHANK3 is phosphorylated by MAPK1 at 18 residues, 3 of which have also been observed in vivo and shown to increase SHANK3 turnover and … Webb功能: Adapter protein that binds F-actin and DNM1, and thereby plays a role in receptor-mediated endocytosis. Plays a role in the reorganization of the actin cytoskeleton, formation of cell projections, such as neurites, in neuron morphogenesis and synapse formation via its interaction with WASL and COBL.
Webb22 maj 2024 · Shank3 is a highly phosphorylated protein. Phosphorylation is one of the most common regulatory mechanisms to modulate synaptic protein interactions and … Webb29 apr. 2024 · Using a parallel proteomic analysis performed on Shank3 isolated from rat neocortical neurons by immunoaffinity, we identified two sites that were hypo-phosphorylated during scaling up and hyper-phosphorylated during scaling down: one (rat S1615) that corresponded to S1539 in mouse, and a second highly conserved site, rat …
Webb26 mars 2024 · We found that both PKA and CaMKII can phosphorylate a GST-Shank3 fusion protein containing residues 572–691, and that this phosphorylation is prevented by mutation of Ser685 to a phospho-null alanine residue. Consistent with prior studies, any mutation of Ser685 disrupts Shank3 interactions with ABI1. However, we show for the … Webb22 nov. 2024 · For example, the interaction between SHANK3 and ABI1 is regulated by phosphorylation at S685, a residue in the proline-rich region (PP) domain, and an ASD-linked patient mutation S685I interferes with this phosphorylation, abolishing interaction with ABI1 and decreasing downstream actin polymerization. 17 However, we have thus …
Webb9 juli 2024 · Shank3 mutation leads to changes in the SNO-proteome of 6w and 4 m InsG3680 (+/+) mice. The SNO-proteomic analysis revealed differences in SNO-proteins …
Webb7 okt. 2011 · Shank3/PROSAP2 gene mutations are associated with cognitive impairment ranging from mental retardation to autism. Shank3 is a large scaffold postsynaptic density protein implicated in dendritic spines and synapse formation; however, its specific functions have not been clearly demonstrated. dodson and horrell dog foodWebb18 okt. 2024 · SHANK3 deficiency results in the upregulation of Cdc2like kinase 2 (CLK2) expression, then lead to the phosphorylation and activation of serine/threonine protein … dodson and horrell echinaceaWebbA bidirectional switch in the Shank3 phosphorylation state biases synapses toward up or down scaling Authors Chi-Hong Wu 1 , Vedakumar Tatavarty 1 , Pierre M J Beltran 2 , … eye doctors rumford maineWebbShank1 and Shank3 contain several RSK consensus phosphorylation sites (RXRXXS, where X is any amino acid) and both Shanks were efficiently phosphorylated by RSK2 in vitro (data not shown). Indeed, RSK2 phosphorylated Shank1 (368–932) with a K m value (0.17 μM) far below that for the S6 peptide (9.6 μM), a standard RSK2 in vitro substrate, … eye doctors schenectady nyWebb16 aug. 2024 · Shank3 is essential for synaptic scaling up, as previously demonstrated by Turrigiano and her colleagues (Tatavarty et al., Neuron, 2024). In their recent study, they … eye doctors scottsburg indianaWebb26 apr. 2024 · Using a parallel proteomic analysis performed on Shank3 isolated from rat neocortical neurons by immunoaffinity, we identified two sites that were persistently hypo-phosphorylated during scaling... dodson and horrell digestive supportWebb4 mars 2024 · The Shank3 shRNA-resistant construct (Shank3 R) was designed by introducing 6 “silent” nucleotide mutations in the target site that do not alter amino acid sequence at Arg 1187, Lys 1188, Ser 1189, and Pro 1190. The mAp-Shank3 construct was generated by inserting Shank3 cDNA into pmApple-C1 construct at BglII and EcoRI … dodson and horrell goat mix