Psicheck-2 promega
Web将细胞用细胞裂解液裂解后，按promega双荧光报告系统测量试剂盒说明取5ul细胞裂解液与萤火虫荧光素酶缓冲液及底物5ul混匀测量荧光强度，随后取海肾荧光素酶反应缓冲液和5ul腔肠素底物混匀后第二次测得海肾荧光素酶的活性。 ... 将目的片段与psiCHECK-2载体连接 ... WebpsiCHECK-2 Vector, 20 µg, Promega Supplier: Promega psiCHECK-1 and psiCHECK-2 Vectors enable monitoring of changes in expression of a target gene fused to a reporter gene. They are used for optimizing RNA interference assays. Vectors for Optimizing RNA Interference Assays
Psicheck-2 promega
Did you know?
WebpsiCHECK™-2 Vector 20μg C8021 Storage Conditions:Store the psiCHECK™-1 and psiCHECK™-2 Vectors at –20°C. Promega Corporation ·2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526· Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TB329 Printed in USA. Page 2 Revised 6/09 WebThis firefly reporter cassette has been specifically designed to be an intraplasmid transfection normalization reporter; thus when using the psiCHECK™-2 Vector, the Renilla luciferase signal can...
WebNational Center for Biotechnology Information WebNov 3, 2024 · psiCHECK™-2 Vector（C8021）最初是为验证 siRNA 靶标设计的。 该载体用海肾萤光素酶（hRluc）作为主报告基因；萤火虫萤光素酶（hluc+）则作为内参 报告基因，用于进行归一化处理。 目的基因被克隆至海肾萤光素酶翻译终止密码子下游的多克隆位点。 由合成的 siRNA 或体内表达的 shRNA 引发的针对目的基因的 RNAi 过程，导致融合 …
WebDual Luciferase Reporter Vector Psicheck 2, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, … WebpsiCHECK2 (Search Vector Database) Backbone manufacturer Promega Backbone size (bp) 6273 Modifications to backbone Insertion of a SalI/NotI/XhoI linker between the NotI and XhoI site Vector type Mammalian Expression Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number
WebInstructions for Use of Product (s)C8021. The psiCHECK™-1 Vector and psiCHECK™-2 Vector provide a quantitative and rapid approach to optimize RNA interference (RNAi). …
WebThe psiCHECK™-2 Vector is designed to provide quantitative and rapid optimization of RNA interference (RNAi). The vector enables monitoring of changes in expression of a target gene fused to a reporter gene. Renilla luciferase is used as the primary reporter gene, and the gene of interest is cloned into a multiple cloning region located ... how to edit images for sublimationWebThe psiCHECK™-2 Vector is designed to provide quantitative and rapid optimization of RNA interference (RNAi). The vector enables monitoring of changes in expression of a target gene fused to a reporter gene. Renilla … led colored can lightsWebHere we demonstrate the utility of the psiCHECK™-2 Vector, originally designed for screening siRNA silencing efficiency, as a biosensor for microRNA activity in cells. Promega Notes 99, 16–18. Article MicroRNA-Biosensors-Application-for-the-psiCHECK-2-Vector PDF (184 KB) – English Related Products psiCHECK™-2 Vector Related Protocols led colored tree lightsWeb主要试剂：BCA蛋白浓度检测试剂盒、RIPA(强)蛋白裂解液、山羊抗兔二抗(中国碧云天生物技术研究所)，性别决定区Y框蛋白5(sex determining region Y-box protein 5, SOX5)抗体、兔抗人甘油醛-3-磷酸脱氢酶(glyceraldehyde-3-phosphate dehydrogenase, GAPDH)抗体(美国Abcam公司)，psiCHECK-2荧光 ... how to edit image in design spaceWebAnalyze Sequence: psiCHECK-2. Sign Up for Our Newsletter. Receive the latest news, hot plasmids, discounts and more. Sign Up. Subscribe to Our Blog. Learn about the latest plasmid technologies and research tools. Subscribe. Contact Addgene. Have questions about your order, deposit, or a plasmid? how to edit image in paintWebPsicheck 2 Luciferase Reporter Vector, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … led colorfulWebpsiCHECK-1 and psiCHECK-2 Vectors enable monitoring of changes in expression of a target gene fused to a reporter gene. They are used for optimizing RNA interference … how to edit image in figma