Cry2 oligomerization
WebMar 2, 2024 · CRY2 photooligomerization occurred rapidly, reaching 50% saturation in approximately 8–15 s in HEK293 cells irradiated with 100 μmol m −2 s − 1 blue light ( … WebThe CRY2-based oligomerization is ideal to recapitulate the luminal domain oligomerization during CRAC channel activation. The PHR domain of CRY2 is used to replace the luminal and transmembrane domains of STIM1 to generate CRY2-STIM1 chimeras (Opto-CRAC2) ( Ma et al., 2024, 2024; Zhao et al., 2011) ( Fig. 1 ).
Cry2 oligomerization
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WebCRY2, a blue-light-sensitive photosensor, interacts with CIB1 in its photoexcited state to form a heterodimer on a sub-second timescale, which is reversible within minutes in … WebWhile CRY2 homo-oligomerization and CRY2-CIB1 heterodimerization could happen concomitantly, the presence of certain CIB1 fusion proteins can suppress CRY2 homo-oligomerization. However, the homo-oligomerization of cytoplasmic CRY2 can be significantly intensified by its recruitment to the membrane via interaction with the …
WebD. Coefficient of variation (CV) analysis of AIM2 PYD-Cry2, directly oligomerized by the blue light pulse and of Doxycycline-inducible ASC-mScarlet not interacting with the optogenetic cluster directly, in engineered THP-1 cells. ... On demand MyD88 oligomerization is controlled by IRAK4 during Myddosome signaling. Web2 hours ago · Moreover, overexpression of CRY2 or deficiency of PIF4/5 repressed the hypocotyl elongation induced by NF-YC7 under blue light. Further investigation revealed …
Webbluelight,cryptochrome,CRY1,CRY2,photoreceptor, photomorphogenesis,transcription,proteolysis,protein–protein interactions,Arabidopsis Abstract Cryptochromes are blue-light receptors that mediate photoresponses in plants. The genomes of most land plants encode two clades of cryp- http://www.cchem.berkeley.edu/schaffer/2013%20Publications/Bugaj_Cry2Opto_NMeth_13.pdf
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WebAn Optogenetic-Controlled Cell Reprogramming System for Driving Cell Fate and Light-Responsive Chimeric Mice. Adv Sci (Weinh), 2024 Precision super-resolution cryo-correlative light and electron microscopy for rapid in situ structural analyses of optogenetically-positioned organelles. bioRxiv, 2024 the police manager 8th editionWebNov 20, 2024 · The blue-light receptor cryptochrome (CRY) in plants undergoes oligomerization to transduce blue-light signals after irradiation, but the corresponding molecular mechanism remains poorly understood. Here, we report the cryogenic electron microscopy structure of a blue-light-activated CRY2 tetramer a … the policeman on the beat moved up the avenueWebThe Dual Characteristics of Light-Induced Cryptochrome 2, Homo-oligomerization and Heterodimerization, for Optogenetic Manipulation in Mammalian Cells. ACS Synth Biol, 2015 Regulation of endogenous transmembrane receptors through optogenetic Cry2 clustering. Nat Commun, 2015 sidify version historyWebOptimized second-generation CRY2-CIB dimerizers and photoactivatable Cre recombinase. Nat Chem Biol, 2016 Optogenetic oligomerization of Rab GTPases regulates … the policeman\u0027s lineage asianwikiWebFeb 28, 2024 · Upon exposure to blue light, the monomeric inactive CRYs undergo phosphorylation and oligomerization, which are crucial to CRY function. Both the N- … sidify torrentWebJun 23, 2024 · Here, we identify important determinants for efficient cryptochrome 2 clustering and introduce a new CRY2 module, named ‘‘CRY2clust’’, to induce rapid and efficient homo-oligomerization of... Metrics - Optogenetic protein clustering through fluorescent protein ... - Nature the policeman stoppedWebSep 15, 2024 · Double-positive charges at residues 489 and 490 enhance CRY2 homo-oligomerization. a A series of mutations were introduced at positions 489 and 490 of CRY2. b Cytosolic CRY2(R489D) and CRY2(R489E ... the policeman caught the thief by the arm